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June 2017 (published: 27.06.2017)
Number 2(32)
Home > Issue > Reflection spectroscopy in the study of muscle tissue of animal origin. Part I
Alla P. Nechiporenko, Uspenskaya Maya Valerievna, Orehova S.M., Liudmila V. Plotnikova, Petr P. Plotnikov, Ishevskii A.L.
The article deals with the first comparative research concerning muscular tissue of animal origin and the impact of consecutive water, salt and alkali extractions on the spectral characteristics of the surface of its main components - the muscle fibers, the stroma, the contracting proteins, and proteins of the stromaby electron spectroscopy of diffuse reflection (ESDO) and infrared spectroscopy of frustrated total internal reflection (FTIR X).The impact dispersion, low negative temperatures (-18°C) and microbial processes were also analyzed. The novelty of this research is the use of differences in solubility of muscle tissuemain components, used for the study of biological materials by spectral methodsfor the first time. The data obtained allowed to identify the bands of stretching vibrations for carbonyl groups of protein, lipid and the structural elements of mucopolysaccharidesin the IR spectra of muscle tissue and compare them with the results obtained by the ESDОmethod that was the purpose of this experiment.
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Keywords: reflection spectroscopy; electronic and infrared spectroscopy; the muscle tissue of animal origin; biological materials.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
UDC 543.424.4
Reflection spectroscopy in the study of muscle tissue of animal origin. Part I
The article deals with the first comparative research concerning muscular tissue of animal origin and the impact of consecutive water, salt and alkali extractions on the spectral characteristics of the surface of its main components - the muscle fibers, the stroma, the contracting proteins, and proteins of the stromaby electron spectroscopy of diffuse reflection (ESDO) and infrared spectroscopy of frustrated total internal reflection (FTIR X).The impact dispersion, low negative temperatures (-18°C) and microbial processes were also analyzed. The novelty of this research is the use of differences in solubility of muscle tissuemain components, used for the study of biological materials by spectral methodsfor the first time. The data obtained allowed to identify the bands of stretching vibrations for carbonyl groups of protein, lipid and the structural elements of mucopolysaccharidesin the IR spectra of muscle tissue and compare them with the results obtained by the ESDОmethod that was the purpose of this experiment.
Read the full article
Keywords: reflection spectroscopy; electronic and infrared spectroscopy; the muscle tissue of animal origin; biological materials.
DOI 10.17586/2310-1164-2017-10-2-29-39
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License