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September 2024 (published: 26.09.2024)
Number 3(61)
Home > Issue > "Alcoholic" fermentation of tobacco in oak barrels. Part 2. Quantitative change in chlorogenic acid and its determination by HPLC
Moiseev Igor V. , Karmanov Denis A., Leznyy Valerii V. , Bondarenko Dmitriy V. , Sinchin Gregoriy P.
We studied the quantitative change in chlorogenic acid (CGA) in raw tobacco during its fermentation in oak barrels in a gas-liquid medium of cognac distillate for six months. Cuban cigar tobacco of the HP variety was used as the object of research. Quantitative determination of CGA was carried out on a liquid chromatograph with a spectrophotometric detector using the external standard method in gradient elution mode. An external standard of CGA was obtained from green coffee beans by extraction with a 40% aqueous solution of methanol, followed by further treatment of the extract with Carrese solutions and evaporation of the treated extract on a rotary evaporator under vacuum. As a result of chromatography of the external standard, a chromatogram in the form of a double peak was obtained. To analyze the tobacco samples under study, their water-methanol extracts were first obtained using the Soxhlet method. HPLC results showed that the mass fraction of CGA in unfermented tobacco raw materials was 0,29%. In fermented tobacco, the content of CGA was 0,19%, which corresponds to a loss of CGA during fermentation by 42,9%. To identify the possible diffusion of CGA from tobacco raw materials into oak staves, a similar analysis was carried out on 1 mm thick oak chips cut from the inner surface of the barrel before and after the fermentation process. Chromatography results showed the absence of CGA in oak chips cut before tobacco fermentation. The chromatogram of the extract of oak chips cut after fermentation showed a peak corresponding to CGA in an amount of 0,047%. However, its shape do not allow us to unequivocally state that this peak directly relates to CGA.We studied the quantitative change in chlorogenic acid (CGA) in raw tobacco during its fermentation in oak barrels in a gas-liquid medium of cognac distillate for six months. Cuban cigar tobacco of the HP variety was used as the object of research. Quantitative determination of CGA was carried out on a liquid chromatograph with a spectrophotometric detector using the external standard method in gradient elution mode. An external standard of CGA was obtained from green coffee beans by extraction with a 40% aqueous solution of methanol, followed by further treatment of the extract with Carrese solutions and evaporation of the treated extract on a rotary evaporator under vacuum. As a result of chromatography of the external standard, a chromatogram in the form of a double peak was obtained. To analyze the tobacco samples under study, their water-methanol extracts were first obtained using the Soxhlet method. HPLC results showed that the mass fraction of CGA in unfermented tobacco raw materials was 0,29%. In fermented tobacco, the content of CGA was 0,19%, which corresponds to a loss of CGA during fermentation by 42,9%. To identify the possible diffusion of CGA from tobacco raw materials into oak staves, a similar analysis was carried out on 1 mm thick oak chips cut from the inner surface of the barrel before and after the fermentation process. Chromatography results showed the absence of CGA in oak chips cut before tobacco fermentation. The chromatogram of the extract of oak chips cut after fermentation showed a peak corresponding to CGA in an amount of 0,047%. However, its shape do not allow us to unequivocally state that this peak directly relates to CGA.
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Keywords: raw tobacco; "alcoholic" fermentation; polyphenols; chlorogenic acid; extraction; high performance liquid chromatography
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
UDC 663.97:543.544.5
"Alcoholic" fermentation of tobacco in oak barrels. Part 2. Quantitative change in chlorogenic acid and its determination by HPLC
We studied the quantitative change in chlorogenic acid (CGA) in raw tobacco during its fermentation in oak barrels in a gas-liquid medium of cognac distillate for six months. Cuban cigar tobacco of the HP variety was used as the object of research. Quantitative determination of CGA was carried out on a liquid chromatograph with a spectrophotometric detector using the external standard method in gradient elution mode. An external standard of CGA was obtained from green coffee beans by extraction with a 40% aqueous solution of methanol, followed by further treatment of the extract with Carrese solutions and evaporation of the treated extract on a rotary evaporator under vacuum. As a result of chromatography of the external standard, a chromatogram in the form of a double peak was obtained. To analyze the tobacco samples under study, their water-methanol extracts were first obtained using the Soxhlet method. HPLC results showed that the mass fraction of CGA in unfermented tobacco raw materials was 0,29%. In fermented tobacco, the content of CGA was 0,19%, which corresponds to a loss of CGA during fermentation by 42,9%. To identify the possible diffusion of CGA from tobacco raw materials into oak staves, a similar analysis was carried out on 1 mm thick oak chips cut from the inner surface of the barrel before and after the fermentation process. Chromatography results showed the absence of CGA in oak chips cut before tobacco fermentation. The chromatogram of the extract of oak chips cut after fermentation showed a peak corresponding to CGA in an amount of 0,047%. However, its shape do not allow us to unequivocally state that this peak directly relates to CGA.We studied the quantitative change in chlorogenic acid (CGA) in raw tobacco during its fermentation in oak barrels in a gas-liquid medium of cognac distillate for six months. Cuban cigar tobacco of the HP variety was used as the object of research. Quantitative determination of CGA was carried out on a liquid chromatograph with a spectrophotometric detector using the external standard method in gradient elution mode. An external standard of CGA was obtained from green coffee beans by extraction with a 40% aqueous solution of methanol, followed by further treatment of the extract with Carrese solutions and evaporation of the treated extract on a rotary evaporator under vacuum. As a result of chromatography of the external standard, a chromatogram in the form of a double peak was obtained. To analyze the tobacco samples under study, their water-methanol extracts were first obtained using the Soxhlet method. HPLC results showed that the mass fraction of CGA in unfermented tobacco raw materials was 0,29%. In fermented tobacco, the content of CGA was 0,19%, which corresponds to a loss of CGA during fermentation by 42,9%. To identify the possible diffusion of CGA from tobacco raw materials into oak staves, a similar analysis was carried out on 1 mm thick oak chips cut from the inner surface of the barrel before and after the fermentation process. Chromatography results showed the absence of CGA in oak chips cut before tobacco fermentation. The chromatogram of the extract of oak chips cut after fermentation showed a peak corresponding to CGA in an amount of 0,047%. However, its shape do not allow us to unequivocally state that this peak directly relates to CGA.
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Keywords: raw tobacco; "alcoholic" fermentation; polyphenols; chlorogenic acid; extraction; high performance liquid chromatography
DOI 10.17586/2310-1164-2024-17-3-3-9
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License