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June 2024 (published: 18.06.2024)
Number 2(60)
Home > Issue > "Alcoholic" fermentation of tobacco in oak barrels.
Part 1. Tannin mass transfer and its indirect quantitative determination by HPLC
Moiseev Igor V. , Karmanov Denis A., Leznyy Valerii V. , Bondarenko Dmitriy V. , Sinchin Gregoriy P.
The quantitative transition of oak tannin into raw tobacco during its fermentation in oak barrels in the gas-liquidmedium of cognac distillate was studied. The object of research was Cuban cigar tobacco of the HP variety. The fermentation process was carried out for six months with periodic (1–2 weeks) treatment of the inner surface of the barrel with cognac distillate with an alcohol content of 70 vol.% in an amount of 1,5 cm3/dm2. To obtain data on tannin mass transfer, we developed a method for the quantitative indirect determination of tannin in oak and tobacco raw materials using reverse-phase high-performance liquid chromatography (RP HPLC) through preliminary hydrolysis of tannin to gallic acid. The determination of gallic acid/tannin was carried out on a liquid chromatograph with a spectrophotometric detector (UV) at a wavelength of 280 nm using the external standard method. The analysis of unfermented tobacco raw materials did not reveal the presence of tannin. It has been shown that under the specified conditions, during 6 months of fermentation, diffusion of tannin from oak staves into tobacco is observed in an amount of 0,11% by weight of tobacco. The mass fraction of tannin in oak staves was 0,81%. At the same time, the most intense saturation occurs in the 1st, 4th, and 5th months of fermentation.
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Keywords: tobacco raw materials; fermentation; cognac distillate; tannin of oak; gallic acid; high-performance liquid chromatography
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
UDC 663.97:543.544.5
"Alcoholic" fermentation of tobacco in oak barrels.
Part 1. Tannin mass transfer and its indirect quantitative determination by HPLC
The quantitative transition of oak tannin into raw tobacco during its fermentation in oak barrels in the gas-liquidmedium of cognac distillate was studied. The object of research was Cuban cigar tobacco of the HP variety. The fermentation process was carried out for six months with periodic (1–2 weeks) treatment of the inner surface of the barrel with cognac distillate with an alcohol content of 70 vol.% in an amount of 1,5 cm3/dm2. To obtain data on tannin mass transfer, we developed a method for the quantitative indirect determination of tannin in oak and tobacco raw materials using reverse-phase high-performance liquid chromatography (RP HPLC) through preliminary hydrolysis of tannin to gallic acid. The determination of gallic acid/tannin was carried out on a liquid chromatograph with a spectrophotometric detector (UV) at a wavelength of 280 nm using the external standard method. The analysis of unfermented tobacco raw materials did not reveal the presence of tannin. It has been shown that under the specified conditions, during 6 months of fermentation, diffusion of tannin from oak staves into tobacco is observed in an amount of 0,11% by weight of tobacco. The mass fraction of tannin in oak staves was 0,81%. At the same time, the most intense saturation occurs in the 1st, 4th, and 5th months of fermentation.
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Keywords: tobacco raw materials; fermentation; cognac distillate; tannin of oak; gallic acid; high-performance liquid chromatography
DOI 10.17586/2310-1164-2024-17-2-3-9
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License