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2014 (published: 24.02.2014)
Number 1(19)
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Applikation cryoprotectans as a nuclaator ice storage fruit crops liguid nitrogen vapor
Pavlov Alexander V , Verzhuk V.G.
Keywords: large fruit –cherry, buds, cryopreservation, cryoprotectors, genofond.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
Applikation cryoprotectans as a nuclaator ice storage fruit crops liguid nitrogen vapor
The influence ultra low temperatures on viability buds fruit-trees after prolonged storage in steams of liquid nitrogen was studied. Processing cherry buds before deep freezing in liquid nitrogen with different composition cryoprotectors permit to receive viable plants after defreezing and germination on the light in vitro. This process takes into account the positive role of cryoprotectants with which you can accumulate free water from the cell into the extracellular environment and thereby reduce the destructive effect of ice formed during freezing and preparing genoplazmy plants to long-term storage in nitrogen vapor. Application cryoprotectants kidney cherry shows that more effectively on the studied varieties protector acts on the basis of 40% sucrose minutes. Treated them cherry varieties kidney Vstrecha after storage in nitrogen vapor showed 60.0% viability, kidney Chudo- cherry - 63.2%. Cryopreservation method for buds by using cryoprotective treatment is perspective and effective. This method should be developed for evaluation of the samples from current genofonds collections. Such treatment stabilizes intracellular metabolism, cryoprotectors transplace free water from cell to intracellular space and thus cryoprotectors become nuclaator ice and protect cells from damage.
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Keywords: large fruit –cherry, buds, cryopreservation, cryoprotectors, genofond.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License